摘要
目的 探讨B7共刺激分子在人类恶性血液病细胞系中的表达及意义。方法 用质粒DNA的扩增、脂质体介导法转基因 ,流式细胞术、RT PCR等方法检测U937、K5 6 2、CEM、DOUDI、GM、PEER、Jurkat、Raji等细胞转基因前后B7 1分子的表达。结果 U937、K5 6 2、CEM、DOUDI、GM、PEER、Jur kat、Raji等 8种恶性血液病细胞株均表达或高表达HLA抗原和B7 2分子 ,低表达或不表达B7 1分子 ,转基因后细胞株B7分子的表达明显增强 ,转B7 1基因后肿瘤细胞刺激T细胞表达高水平的IL 2mRNA。结论 大多数人类恶性血液病细胞株均不表达或低表达B7分子 ,提示B7 1可能在肿瘤免疫中起主要作用。
Objective To explore the expression of B7 co stimulator in human malignant hematological cell lines and its significance. Methods Amplified and purified plasmid DNA was transfected by liposome and detection of B7 gene expression by flow cytometry and RT PCR. Result Eight human malignant hematological cell lines U937,K562,CEM,Daudi,GM, PEER, Jurkat,Raji express high level of HLA molecules, low or undetectable level of B7 1 gene,and high level of B7 2 gene. Expression level of B7 1 gene was enhanced after B7 1 transfection. T cell expressed high level of IL 2 mRNA after B7 1 transfection. Conclusion Multiple human malignant hematological cell lines express low or undetectable level of B7 1 gene, suggesting that B7 1 plays a critical role in tumor immunity.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2002年第7期345-348,共4页
Chinese Journal of Hematology
基金
国家自然科学基金资助项目 (3 9770 3 2 8)
