摘要
根据报道的大肠杆菌 (Escherichia coli)海藻糖 6磷酸合酶基因 (ots A) ,设计引物 ,通过 PCR技术从大肠杆菌 XLI菌株的总 DNA中扩增到一个 1 .4kb的片段。经克隆、测序分析 ,该片段长 1 42 5 bp并含一完整的开放读框 (ORF)。在核苷酸水平上 ,该 ORF与已报道的 ots A基因具有 99.86%的同源性。在氨基酸水平上 ,其推断性的编码产物蛋白与 Ots A具有 1 0 0
A 1 4 kb DNA fragment was amplified by PCR from total DNA of Escherichia coli XLI using 2 primers which were designed according to the reported trehalose6phosphate synthase gene otsA .The results of sequencing analyses showed that the fragment has an open reading frame(ORF) containing 1 425 nucleotides, which shared a homology of 99 86% with E.coli SCH7 otsA gene at the nucleotide level, and the putative protein shared an identity of 100% with the protein OtsA encoded by otsA gene at the amino acid level.
出处
《广西农业生物科学》
CAS
CSCD
2002年第2期98-100,共3页
Journal of Guangxi Agricultural and Biological Science
基金
国家自然科学基金资助项目 (3 0 1 60 0 45 )
