摘要
以海藻糖高产菌株啤酒酵母SaccharomycescerevisiaeAS2.1416的cDNA克隆为模板 ,根据国外报道的序列 ,设计适当的引物 ,利用PCR技术 ,克隆出大约1.5kb的片段。PCR产物经低溶点胶纯化 ,与pBluscript载体连接并转化大肠杆菌DH -5α ,阳性重组子经酶切鉴定 ,表明已获得海藻糖磷酸酶基因PCR产物及其克隆。
Based on template of Saccharomyces cerevisiae cDNA and reported gene sequence, a pair of primers was designed to amplify a 1.5kb DNA fragment by using PCR. The PCR product was purified on 1% LMP agarose gel, and cloned into plasmid pBluscript, followed by transformation of R.coli DH-5α. The Cloned PCR product was confirmed to be TPS1 by restriction endonuclease.
出处
《沈阳农业大学学报》
CAS
CSCD
2000年第4期334-336,共3页
Journal of Shenyang Agricultural University
