摘要
采用正交试验设计方法研究了MS微量元素、2 ,4 D、萘乙酸、脯氨酸和腺嘌呤的浓度对 5种基因型大豆未成熟子叶愈伤形成和体细胞胚胎发生的影响。结果表明 ,MS微量元素的浓度对出愈率和胚胎发生率均有显著的作用 ;在MS培养基中附加 2 ,4 D、萘乙酸、脯氨酸和腺嘌呤对愈伤的形成有明显的促进作用。微量元素浓度对大豆体细胞胚胎发生有明显的影响 。
The effects of the concentration of MS micro salts, 2,4 dichlorophenoxyacetic acid(2,4 D), naphthalene acetic acid (NAA), proline and adenine on callus formation and embryogenesis were investigated using orthogonal design with immature cotyledon of soybean. The results showed that the role of concentration of micro salts on frequency of callus formation and embryogenesis were significant. MS medium plused 2,4 D, NAA, proline or adenine can stimulate callus formation. The concentration of MS micro salts possessed obvious effects on somatic embryogenesis of soybeans. The category and concentration of the hormone needed were different among genotypes when embryogenesis are induced.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第6期606-609,共4页
Scientia Agricultura Sinica
基金
国家植物转基因中试及产业化基地专项基金 (J99 B 0 0 1)
