摘要
染料木素是大豆发酵食品中的主要异黄酮成分。制备了牛血清白蛋白-染料木素免疫抗原和卵白蛋白-染料木素包被抗原,并对它们进行紫外和SDS-聚丙稀酰胺凝胶电泳鉴定。抗染料木素抗体的效价为1:1600。建立竞争ELISA测定发酵大豆制品中染料木素含量方法,染料木素测定浓度范围为6.25～150μg/L;回收率为87%～102%;亲和常数为1.62×108;与大豆甙元、芒柄花素的交叉反应率分别为9.8%、4.57%;与槲皮素、儿茶素、单宁和核黄素等结构类似物无交叉反应。同时将ELISA法与薄层层析法进行比较。
Genistein was the main component of i soflavone in the fermented soybean f ood.The immune antigen of BSA -genistein andthe coatedantigenof OA -genistein were synthesizedr espectively,andidentified with ul traviolet absorptionspectrometryandSDS -polyacrylamidegelelectrophoresis.TheantibodyIgGofantigenisteinwa spuredwith saturatedsolutionof(NH 4 ) 2 SO 4 andthe columnchromatography ofSep hadex G -25,with1:1600titer ofanti body IgG of antigenistein.The competitive ELISAfor detecting the genistein con tent in the fermented soybean food wa s devel-oped,with6.25～150μg /Lofthedetectingconcentrationr angeofgenistein,therecoveryof87%～102%,theaffinity constant of 1.62×10 8 ,and the 9.8%and 4.57%cross reactio n ratios of antibody for daidzin and f ormononetin re-spectively,while no cross reaction for catechin,tannin,quercetin and riboflavin was observed.The ELISAmethodand thin -leaver chromatography method were also compared for the effect of assaying genistein.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第7期118-120,共3页
Food Science
