摘要
以牛血清白蛋白（ＢＳＡ）为载体，合成２种不同的物质的量的比值（约１∶１３和１∶４２）的磺胺甲基嘧啶抗原，并比较了两者的免疫原性；以卵白蛋白（ＯＡ）为载体，合成１种包被抗原；用辣根过氧化物酶标记羊抗兔ＩｇＧ，工作浓度１∶１０００；建立的乳中磺胺甲基嘧啶残留ＥＬＩＳＡ测定法，抗原亲和常数分别为１．２８×１０７（１∶４２）和３．２１×１０７（１∶１３），磺胺甲基嘧啶检测质量浓度为５～２２０μｇ／Ｌ（１．８７×１０－８～８．２×１０－７ｍｏｌ／Ｌ），检测限为２．４μｇ／Ｌ，回收率为８８％～１１８％，与磺胺二甲基嘧啶和磺胺噻唑交叉率分别为６．０％和０．８４％。
Two kinds of the sulfamethyldiazine antigen were synthesised with the carrier protein of BSA or OA and their immunogenicity compared; the sheep anti rabbit γ globulin were labelled with the horseradish peroxidase (HRP) according to sodium periodate method; the ELISA on sulfamethyldiazine content in milk was developed. Affinity constant of the antibody was 1.28×10 7 (the molecular mole ratio in antigen for 1∶42) and 3.21×10 7(1∶13), respectively. The detectable concentration of sulfamethyldiazine with ELISA revealed 5 220 μg/L (1.87×10 8 8.2×10 7 mol/L) with the detection limit being 2.4 μg/L,the recovery rate of 89% 118%,the cross reactive rate with sulfadimidine and sulfathiazole of 6.0% and 0.84%, respectively, no cross reaction with sulfadimethoxine and sulfaguaniline.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1999年第2期175-177,共3页
Chinese Journal of Veterinary Science
基金
国家青年科学基金
