摘要
采用氨甲喋呤或胸苷阻断法使细胞分裂同步化,并结合胰酶G-带技术,对中国7个家猪品种高分辨G-带进行了研究,发现家猪品种间带型基本一致,从而参照人类细胞遗传学命名法的国际体制,提出了中国家猪高分辨G-带标准化核型及模式图,对显带核型界标进行了少许修改,对每对染色体进行了区带划分和描述。单倍染色体组所显示的G-带数目,包括X和Y染色体,巳达444条,近于中期染色体带纹数目的两倍。
Lising methotrexate or thymidine treatment to induce synchronous division, combined with GTG technique, the high resolution banded chromosomes of seven domestic pig breeds of China were investigated. No distinct difference was found among then. Hence, in line with The Internatioal System for Human Cytogenetic Nomenclature, a standard idiogram of high resolution G-banded karyotype of domestic pig is suggested in this paper. In the idiogramma-tic presentation of G-banding pattern, all the regions and bands of each pair of chromosomes are designated and described with slight modifications for the landmark system. Up to 444 bands were detected per haploid set, including the X and Y chromosomes, a number nearly two times as much as that in banded metaphase chromosomes.
基金
国家自然科学基金
关键词
家猪
染色体
高分辨G带
Domestic pig, Chromosome, High resolution G-band
