摘要
采用淋巴细胞培养和银染法对我国地方猪种太湖猪的4大类群(二花脸猪、枫泾猪、嘉兴黑猪、梅山猪)和姜曲海猪及国外猪种大约克夏猪、丹麦长白猪的银染核仁组成区(Ag-NOR)的数目,分布和银染联合进行研究。结果表明:猪品种间 Ag-NOR 存在差异,二花脸、枫泾、嘉兴黑、梅山、姜曲海、大约克夏及长白猪每细胞 Ag-NOR 均数分别为3.68,3.65,3.61,3.50,3.23,2.15和2.01;银染联合率分别为0.74%,0.50%,2.82%,4.40%,2.27%,0.28%和2.80%.以 Ag-NOR 的数目和频率计算遗传距离并进行聚类分析,二花脸、枫泾、嘉兴黑和梅山猪为同一组,首先聚类在一起.这为太湖猪的归并提供了细胞遗传学依据。
Blood samples from Chinese native pigs(Erhualian,Fengjing,Jiaxinghei,Meishan,Jiangquhai)and foreign pigs(Large Yorkshire and Landrace)were cultured and stained insilver nitrate to study the number and distribution of their Ag-NOR and silver staining associations.The results were as follows:(1)The mean numbers of Ag-NOR per cell for Erhualian,Fengjing,Jiaxinghei,Meishan,Jiangquhai,Large Yorkshire and Landrace were 3.68,3.65,3.61,3.50,3.23,2.15 and 2.01 respectively,and the frequency of silver staining association were 0.74%,0.50%,2.82%,4.40%,2.27%,0.28% and 2.80% respectively.(2)Erhualian,Fengjing,Jiaxinghei andMeishan clustered together earlier compared with other breeds,providing an evidence that the fourtypes of pigs can be classified into one breed called Taihu.It is suggested that as a genetic remarker,Ag-NOR is of breed characteristic,and can be used to study pig origin and breed classification.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1993年第1期72-77,共6页
Journal of Nanjing Agricultural University
关键词
猪
核仁
银染
太湖猪
pigs
silver staining nucleolus organizer regions(Ag-NOR)
breed classification
