摘要
目的 研究基底外侧杏仁核 (BLN)内一氧化氮 (NO)对大鼠睡眠及行为活动的影响。方法 运用多导睡眠描记术、组织化学、行为活动测定等方法研究观察大鼠的睡眠和行为的变化。结果 (1)一氧化氮合酶 (NOS)抑制剂L 硝基精氨酸(L NNA ,2 .0 μg) ,使慢波睡眠 (SWS)增多 ,δ波幅度增大 (睡眠加深 ) ;NO供体硝普钠 (SNP ,0 .2 μg) ,可引起SWS减少、行为活动增加 ,SNP对睡眠及行为活动的影响均可被鸟苷酸环化酶抑制剂亚甲蓝 (MB)所阻断 ;NO前体L 精氨酸 (L Arg ,0 .4 μg)对睡眠无直接影响 ,但能阻断L NNA的促睡眠效应。(2 )NADPH d组化方法发现 ,剥夺 2 4小时睡眠后大鼠BLN内NOS阳性神经元明显增多且深染。结论 BLN内NO含量增多具有明显的抑制SWS和增加行为活动的作用 ,其机制可能与NO通过激活鸟苷酸环化酶而升高cGMP水平有关。
Objective To study the effects of nitric oxide(NO) in basolateral amygdaloid nuclei(BLN)on sleep and behavioral activity in rats. Methods Polysomnography (PSG) ,behavioral test and histochemistry technique were applied to investigate sleep and the behavioral activity of rats. Results (1) N nitro L arginine (L NNA, 2.0μg), an inhibitor of nitric oxide synthase (NOS), increased the duration and depth of slow wave sleep (SWS).Sodium nitrioprusside (SNP, 0.2μg), the donor of nitric oxide (NO), caused decrease of SWS, enhancement of behavioral activity ;its effects on sleep wake state and behavior could be blocked by methylenblue (MB), an inhibitor of guanylate cyclase. L arginine (L Arg, 0.4μg), a precursor of NO, had no effect on sleep wake state, but it completely blocked the promoting sleep effects of L NNA. (2) Changes of NOS positive neurons in BLA were studied with NADPH diaphorase histochemistry method following 24h sleep deprivation. The results showed that the NOS positive neurons increased markedly and were stained densely as compared with control group. Conclusion Enhancement of content of NO in BLN had distinct SWS inhibiting and behavior promoting roles, which was mediated by activation of guanylate cyclase and increase of cGMP concentration.
出处
《中国行为医学科学》
CSCD
2002年第3期247-249,共3页
Chinese Journal of Behavioral Medical Science
基金
安徽省教育厅自然科学资助项目 (2 0 0 2kj172 )
安徽医科大学青年科研基金
关键词
一氧化氮
睡眠
行为活动
杏仁核
Nitric oxide
Sleep
Behavioral activity
Amygdaloid nuclei
