摘要
目的 探讨T淋巴细胞亚群、可溶性白介素2受体和肿瘤坏死因子α在梅毒发病机制中的作用。方法 应用流式细胞仪和双抗体夹心ELISA法对86例梅毒患者T淋巴细胞亚群（CD3+、CD4+、CD8+）、可溶性白介素2受体（sIL-2R）及肿瘤坏死因子α（TNF-α）水平进行检测,并对其相关性进行分析。结果 梅毒患者CD4+及CD4+/CD8+比值明显低于正常人对照组（P<0.01）,CD8+显著高于正常人对照组（P<0.05）,而CD3+与正常人对照组无显著性差异（P>0.05）;活动期梅毒CD4+/CD8+比值低于恢复期（P<0.05）。活动期梅毒及非活动期梅毒患者血清sIL-2R水平均明显增高（P<0.01）,且活动期高于非活动期（P<0.01）。活动期梅毒患者血清TNF-α水平高于非活动期及正常对照组（P<0.01）,且活动期高于非活动期（P<0.01）。活动期梅毒患者 CD4+、CD8+与 sIL-2R和 TNF-α水平呈正、负相关。结论 本研究提示梅毒患者存在细胞免疫功能抑制现象,且 T淋巴细胞功能的紊乱和TNF-α及sIL-2R水平的改变与梅毒的发病有密切关系。
Objective To investigate the effect of T-lymphocyte subgroups, tumor necrosis factor(TNF-α) and soluble interleukin 2 receptor(sIL-2R) in patients with syphilis. Methods We examined T lymphocyte subgroups, TNF-α and sIL-2R in the peripheral blood of 86 patients with syphilis by flow cytometry and a double-antibody 'sandwich' ELISA. Results It showed that ① the number of CD4+ cells and CD4 + /CD8 + ratio in patients with syphilis was significantly lower than those in the control( P <0.01), the number of CD8+ cells was higher than that in the control(P <0.01), the number of CD3+ cells was no difference in patients and the control( P > 0.05) . CD4 + /CD8 + ratio in the active syphilitic cases was lower than that in the static ones( P <0.05) .②The sIL-2R level of patients with syphilis was significantly higher than the controK P < 0.01), the sIL-2R level of the patients with active syphilis was higher than that of the static cases ( P <0.01).③The level of TNF-α was elevated in patients with active syphilis than the control ( P <0.05).④ It was showed significant positive and negative correlation between T-lymphocyte subgroups, sIL-2R and TNF-α. Conclusion The study suggests that there is prominent suppression of cellular immunity function in the patients with syphilis, and the disorder of T-lymphocyte function, serum levels of sIL-2R and TNF-α were closely associated with mechanism of syphilis.
出处
《中国性病艾滋病防治》
2002年第3期139-141,共3页
Chinese Journal of Std & Aids Prevention and Control
