摘要
目的 观察组氨酸脱羧酶 (Histidine decarboxylase,HDC) m RNA在小鼠三叉神经节中的表达 .方法 应用原位杂交组织化学技术和不同浓度地高辛标记的寡核苷酸探针检测小鼠三叉神经节中 HDC m RNA的表达 .结果 在小鼠三叉神经节的周边部有密集的 HDC阳性神经元 ,而在中间区域仅见散在的 HDC阳性神经元 .HDC主要位于大、中型细胞的胞质内 ,且杂交信号随探针浓度的增加而增强 .结论 由于HDC能催化组氨酸脱羧基形成组胺 ,提示组胺可在三叉神经节细胞的胞质内合成 ,与降钙素基因相关肽 (CGRP)、P物质(SP)等神经递质共存 .合成的组胺在三叉神经支配其效应器过程中和 CGRP。
AIM To study the expression of histidine decarboxylase mRNA in mouse trigeminal ganglia. METHODS Using in situ hybridization histochemistry together with specific oligonucleotide probe, we detected the histidine decar boxylase mRNA hybridization signals in the trigeminal ganglia. RESULTS Histidine decarboxylase mRNA hybridization signals was strong in the peripheral area of the trigeminal ganglia, while some scattered positive cells were also detected in central area . The positive signals were mainly confined to two subpopulation of neurons: large cells and middle cells. CONCLUSION Histidinedecarboxylase is the only enzyme which catalyzes histidine into histamine, so it is regarded as the marker of the histaminergic cell. Therefore, histamine may be synthesized in the trigeminal ganglia and modulates other important neurotransmissions such as CGRP, SP, NPY, participates the normal physiological processes.
出处
《第四军医大学学报》
北大核心
2002年第12期1057-1060,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (3 990 0 0 75 )
关键词
组氨酸脱羧酶
三叉神经节
原位杂交
小鼠
histidine decarboxylase
trigeminal ganglion
in situ hybridization
mice
