摘要
目的 观察大鼠三叉神经中脑核 (Vme)内谷氨酸脱羧酶 (GAD)样阳性终末与磷酸激活的谷氨酰胺酶(PAG)、GABAA 受体α3亚单位 (GABAARα3)和小白蛋白 (PV)样阳性神经元之间的联系。 方法 免疫荧光组织化学三重染色技术 ,在激光共聚焦显微镜下进行观察。 结果 在Vme内可观察到大量的GABAARα3样、PAG样和PV样免疫反应阳性神经元 ,吻尾方向几乎在其全长出现 ,多为大的假单极神经元 (直径为 2 5～ 5 0 μm)。大约 90 %左右的PAG样阳性神经元同时呈GABAARα3样和PV样免疫阳性。在激光共聚焦显微镜下观察到 ,密集分布的GAD样阳性终扣聚集在PAG样和GABAARα3样双重标记的Vme神经元胞体周围 ,并与之形成密切接触。 结论 GA
Objective To observe the connections between the glutamic acid decarboxylase\|like immunoreactive(GAD\|LI) axonal terminals and the positive neurons showing phosphate actived glutaminase(PAG)\|LI or GABA\-A\|receptor α3 subunit (GABA\-ARα3)\|LI and parvalbumin(PV)\|LI in the mesencephalic trigeminal nucleus(Vme) of the rat. Methods The triple\|immunofluoresence histochemical staining techniques were used in the present study,and stained sections were observed under a confocal laser\|scanning microscope. Results Many neuronal cell bodies through the whole rostrocaudal extent of Vme were showed dense GABA\-ARα3\|,PAG\| and PV\|like immunoreactivities,respectively.The majority of them were large pseudounipolar neurons(diameter 25\|50μm).About 90% of PAG\|LI neurons expressed both GABA\-ARα3\|LI and PV\|LI.Confocal laser\|scanning microscope further revealed that dense GAD\|LI axonal varicosities were found to surround the somata of Vme neurons showing both GABA\-ARα3\| and PAG\|like immunoreactivities,and formed the close contact each other.Conclusion\ The present results suggested that the GABAergic axonal terminals projecting to Vme might exert inhibitory regulation and control effect through GABA\-ARα3 subunit localizated in somata of Vme neurons on the transmission of orofacial region proprioceptive sensory information mediated by glutamate.
出处
《解剖学报》
CAS
CSCD
北大核心
2003年第1期6-10,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目 (3 9870 2 62 )
国家教育部高等学校骨干教师资助计划资助项目
