摘要
GC-MS联用分析诺乙龙阳性尿,检出了诺乙龙经人体吸收代谢以后产生的九个代谢产物。除Met-2和Met-7在服药后84h的尿样中仍能被检出以外,诺乙龙原型及其它代谢物的体内存留时间较短,可检测时间不超过35h。通过解析质谱,鉴定了七个代谢物的分子结构,发现A环双键还原和D环羟基化是诺乙龙的两种体内代谢途径.用大孔树脂吸附提取、酶解、衍生化等处理尿样,方法回收率达80%,诺乙龙的检测下限为10ng/ml(10 ppb).
Steroids in human urine were adsorbed on a macroporous XAD-2 resin, eluted with methanol, hydrolyzed with glucuronidase, extracted and concentrated for TMS derivatization and then analyzed with GC-MS. Norethandrolone(N) and its 9 metabolites were detected in urine samples 9～35h after oral administration. Met-2 and Met-7 could be detected even in 84h urine sample. The structures of 7 metabolites were elucidated and the variations of their concentration in urine were determined. The specific metabolites and characteristic ions for screening N positive urine were chosen. This method is sensitive enough; the detection limit of N was 10 ng per ml urine, i. e. 10 ppb.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第10期777-781,共5页
Acta Pharmaceutica Sinica
