摘要
运用薄层层析法及二极管阵列检测器与高效液相色谱联用的方法 ,确定红肉脐橙果肉中含有番茄红素和 β -胡萝卜素两种主要色素 ,它们在液相色谱图中占总峰面积 (计溶剂峰 )的 73 .5 7%～77.78% ,番茄红素占 5 5 .3 6%～ 5 8.49% ,β-胡萝卜素占 1 8.2 1 %～ 1 9.93 %。利用反相高效液相色谱仪以及番茄红素和 β-胡萝卜素标样制作标准曲线 ,测定果实发育及室温贮存过程中色素的变化动态 ,结果表明 ,该脐橙果肉中的番茄红素在成熟期含量最高 ,达 1 0 9.67μg·g-1DM ,β -胡萝卜素此时也达到最高值( 1 5 .5 2 μg·g-1DM) ;番茄红素的含量在采收后基本呈下降趋势 (但采后 3 0d有显著升高 ) ,说明果肉中存在类胡萝卜素采后合成的现象 ;贮存 4个月后 ,番茄红素含量急剧降低至最高时的 1 /1 0。
In this article, two main pigments lycopene and β carotene were identified in Red flesh navel orange by using Thin Layer Chromatography and PAD combined with HPLC, its red flesh was pigmented by lycopene which made up to 55.36%-58.49% of the total peak area(counted in peak area of solvent) , whereas β carotene held 18.12%-19.93%. Using Reverse phase HPLC, standard curves of authentic lycopene and β carotene were obtained and then be applied to calculate concentration changes of pigments during fruit development and storage under room temperature. Results showed that concentration of carotenoids was the highest at ripening time (lycopene was up to 109.67μg·g -1 of dry mass, and β carotene as much as 15.52μg·g -1 ), after harvest, a tendency of decreasing in pigments was easy to be seen, but there was also a significant increase appeared in Jan. 16th. After 4 months of storage, concentration of carotenoids reached the lowest among all investigated periods.
出处
《园艺学报》
CAS
CSCD
北大核心
2002年第3期203-208,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金重点项目
农业部资助项目
关键词
红肉脐橙
果肉
色素
定性
色素含量
薄层层析
高效液相色谱
Red flesh navel orange( Citrus sinensis L.)
Pigments
Thin layer chromatography
High performance liquid chromatography
