摘要
保存不同时间的兔角膜内皮细胞,SEM:六角形边界不清,仅可见细胞轮廓,细胞有水肿,细胞间连接处有空泡。TEM:核膜尚完整,胞浆内有空泡,还有细胞器存在。术后48天及11个月植片,可见六角形边界的内皮细胞,呈镶嵌型排列,细胞核膜完整,细胞器丰富。提示:保存角膜内皮细胞形态学上的改变在活体房水中的再水合作用或培育下是可以恢复的。
Scanning electron microscopy on the endothelial cells of corneas of rabbits preserved various durations revealed that the hexagonal borders were not clear cut, only cellular circumference could be seen. Transmission electron microscopy showed essential complete nuclear membranes and vacuoles and there are organellae within the cytoplasm. Similar examination of the graft using the preserved cornea 48 days and 11 months after keratoplasty showed endothelial cells arranged in mosaics with complete nuclear membrane and abundant organellae. The above observation suggested that the morphological changs on the endothelial cells of the preserved cornea could be recovered after rehydration or cultivation in aqueous humor in vivo.
出处
《眼科研究》
CSCD
1991年第1期5-7,共3页
Chinese Ophthalmic Research
关键词
角膜移植
保存
角膜
超微结构
兔
cornea preservation, cornea endothelium, rabbit, ultrastructure.
