摘要
目的 研究黄芪总提物 (TEA )对体外肝星状细胞增殖和产生胶原的影响。方法 采用IV型胶原酶灌流分离大鼠肝星状细胞进行原代培养 ,用CCl4急性损伤的大鼠肝枯否细胞条件培养基 (KCCM )刺激肝星状细胞 ,用3H TdR和3H Proline的掺入法分别检测肝星状细胞的增殖和胶原的产生。结果 TEA (5～ 4 0mg·L- 1)可明显降低由KCCM刺激的肝星状细胞的增殖和胶原的产生。结论 TEA对体外激活的肝星状细胞的增殖和产生胶原有明显抑制作用 。
Aim To study the effects of total extract of astragalus(TEA) on activating the primary cultured hepatic stellate cells(HSC) and synthesizing collagen. Methods Livers of adult rats were perfused by pronase E and collagenase in situ, HSC and Kupffer cell (KC) were isolated by centrifugation with 18% Nycodenz. The subcultured HSC were induced by Kupffer cell conditioned medium(KCCM) and were incubated with 5～ 80 mg·L -1 TEA for 6 days. The proliferation of the cells was measured by 3H TdR and the production of collagen by 3H Proline.Results TEA (10～ 40 mg·L -1 ) could suppress the proliferation of hepatic stellate cells and TEA(40～ 80 mg·L -1 ) could suppress the production of collagen. Conlusion The suppression of hepatic stellate cell proliferation and the collagen's production by the TEA may be one of the mechanisms for depressing the hepatic fibrosis by the TEA.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2001年第2期111-113,共3页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
黄芪总提物
肝星状细胞
枯否细胞
增殖
胶原
大鼠
total extract of astragalus (TEA)
hepatic stellate cells
kupffer cell
proliferation
collagen
