摘要
目的 :研究CD15mRNA表达与原发性肝细胞癌 (HCC)侵袭转移和预后关系及与CD4 4v6和nm2 3H1的mRNA表达相关性。方法 :应用原位杂交和免疫组织化学方法 ,检测分析HCC组织中CD15mRNA及其蛋白、CD4 4v6及nm2 3H的mRNA表达。结果 :99例HCC中 ,CD15mRNA及其蛋白、CD4 4和nm2 3H1的mRNA表达阳性率分别为 38 4 %、36 7%、4 1 4 %和76 8%。CD15mRNA表达与其蛋白表达一致 ,与CD4 4v6mRNA表达呈正相关 ,与nm2 3H1mRNA表达呈负相关。CD15mRNA及其蛋白、CD4 4和nm2 3H1的mRNA表达均与HCC侵袭转移倾向和患者预后相关。结论 :检测CD15表达有可能成为HCC侵袭转移和预后判断的一项新的病理生物学指标。
Purpose To investigate the association of the expression of CD15 mRNA with the invasion and prognosis of hepatocellular carcinoma (HCC) and the expression of CD44v6 and nm23H1 mRNAs. Methods In situ hybridization and immunohistochemical methods were used to detect the expression of CD15 mRNA and protein, CD44v6 mRNA and nm23H1 mRNA in HCC. Results In 99 cases of HCC, the positive rates of CD15 mRNA and protein, CD44v6 mRNA and nm23H1 mRNA were 38 4%, 36 7%, 41 4% and 76 7%,respectively. The expression of CD15 mRNA was consistent with its protein, positively correlated with the expression of CD44v6 mRNA and negatively with nm23H1 mRNA. The expression of CD15 mRNA and protein, CD44v6 mRNA, nm23H1 mRNA were associated with the invasive and metastatic trend of HCC and the prognosis of HCC patients. Conclusion The detection of CD15 expression can be a new pathological biology indicator to judge the metastasis and prognosis of HCC.
出处
《临床与实验病理学杂志》
CAS
CSCD
2002年第2期167-170,共4页
Chinese Journal of Clinical and Experimental Pathology
