摘要
目的 :探讨端粒酶hTR、hTRT基因表达在人肿瘤中的意义 ,并与 p5 3蛋白改变相比较 ,评估其对肿瘤临床诊断的价值。方法 :用原位杂交及免疫组化技术检测了 115例恶性肿瘤、6 0例良性及癌前病变中hTR和hTRT的表达 ,并与 p5 3基因蛋白改变相比较。结果 :hTR、hTRT在人恶性肿瘤中的平均检出率分别为 81%、83%。在转移性癌中两者均为 10 0 %的阳性率 ,而在慢性肝炎伴肝硬化中hTR、hTRT分别可见 5 0 %、6 0 %的弱阳性表达 ,其他良性病变及癌前病变均为阴性。p5 3蛋白在人恶性肿瘤中的平均阳性率为 4 9 6 % :在良性病变及癌前病变均为阴性。结论 :恶性肿瘤中hTR和hTRT明显高于 p5 3的检出率 ,且在良恶性病变中的表达差异有显著性 (P <0 0 5 ) ,并提示端粒酶hTR、hTRT与癌的生物学行为有密切关系 ,端粒酶基因hTR、hTRT的原位杂交检测可以作为一个比 p5 3更好的肿瘤诊断的新标记物。
Purpose To investigate the expression of telomerase hTR and hTRT gene in human tumors and to compare with accumulation of p53 to determine its value in clinical diagnosis of human tumors. Methods One hundred and fifteen cases of malignant tumors and 60 benign and precancerous lesions were analyzed in hTR and hTRT expression, and p53 alteration by in situ hybridization and immumohistochemistry. Results hTR and hTRT positive rates in human cancers were 81% and 83% on the average and in the metastatic carcinomas reached 100%, respectively. The benign and precancerous lesions were negative for hTR and hTRT while 50%,60% cases of hepatitis and cirrhosis were weakly expressed. The positive rate of p53 in human malignent tumors was about 49 6%, while the benign and precancerous lesions were all negative. hTR and hTRT positive rates in human cancers were significantly higher than that of p53. Conclusion The expression of telomerase hTR and hTRT gene correlate closely with malignant phenotype of human tumors and is more frequent positive than p53, suggesting that hTR and hTRT may be new indicators in cancer diagnosis.
出处
《临床与实验病理学杂志》
CAS
CSCD
2002年第2期156-158,共3页
Chinese Journal of Clinical and Experimental Pathology
基金
山西省自然科学基金资助项目 (No 199910 85 )
