摘要
目的 :探索用组织工程方法构建人工神经的可行性。方法 :用鼠尾胶和层粘蛋白共同修饰的 polyglytin 910纤维以及生物膜作为支架与成年兔雪旺细胞培养 2周。观察细胞生长迁移情况。结果 :雪旺细胞能在共聚物纤维上迁移包裹 ,均匀分布于支架之间及吸附在支架表面。雪旺细胞在生物膜上也能吸附生长。结论 :成年雪旺细胞可以在修饰后的 polyglytin 910纤维和生物膜上得到扩增 。
Objective: To investigate the possibility of fabricating artificial nerves with tissue engineering methods.Method:The Schwann cells were cultured when they were seeded on polyglactin 910 scaffolds and biomembrane coating with rat tail glue and laminin together and respectively for 2 weeks.The observation of the scaffolds'adsorptivity to Schwann cells,the growth and migration of the Schwann cells were made.Result:The Schwann cells could migrate on the fibre and wrapped fibre up with circle.Schwann cell were well distributed.Schwann cell on biomembrane could also grow well.Conclusion:Adult SC can be expanded on the coated fibre and the biomembrane.Three dimension scaffolds with Schwann cell had the basic characteristics of the artificial nerve.
出处
《中国矫形外科杂志》
CAS
CSCD
2002年第5期456-459,共4页
Orthopedic Journal of China
基金
上海市科学技术发展基金 (项目编号 0 1 4 0 1 90 2 8)
国家863启动金 (EXF0 0 0 31 8)
关键词
雪旺细胞
体外培养
生物材料
组织工程
Schwann cell
Cell cultutre in vitro
Biomaterials
Tissue engineering
