摘要
目的 建立胶质瘤诱导分化相关基因表达谱。方法 用苯丁酸钠诱导胶质瘤细胞分化 ,cDNA阵列检测诱导前和诱导 2h、6d后的 180 0 0个基因表达 ,扫描仪分析结果 ,多点杂交验证。结果 在胶质瘤细胞诱导分化后 ,有 98个基因发生表达变化 ,一些转录和翻译系统相关基因和癌基因表达下调 ,而分化、凋亡基因上调。还有 18个未知基因EST片段分化前后表达差异。结论 含有98个基因的胶质瘤细胞诱导分化相关基因谱可为探讨其分化分子机制提供依据。
Objective Establishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible. Method The expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot. Results Ninty-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also. Conclusion A gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2002年第3期222-225,共4页
Chinese Journal of Oncology
基金
国家自然科学基金资助项目 ( 39870 82 6 )
