摘要
目的 探讨DNA修复基因的变化及其在人脑胶质瘤恶性进展过程中的作用。方法 采用cDNA芯片检测同一患者初发和两次复发的胶质瘤组织与正常脑组织中基因表达的差异 ,建立胶质瘤恶性进展相关基因表达谱。根据GeneBank、GeneCards上检索的资料分析其中DNA修复基因的变化。结果 确立与DNA修复相关的基因 17条 ,分别在肿瘤恶性进展的不同阶段上调或下调。结论 一些DNA修复基因可能参与了人脑胶质瘤的放疗。
Objective To study the changes of DNA repair genes in the gene expression profiles and their potential function during malignant progression of gliomas.Methods The different expression of genes in the primary and the recurrent gliomas specimens in the same patient and the normal brain tissue by cDNA microarray techniques was detected and gene expression profiles associated with malignant progression of gliomas were established . The alteration of DNA repair genes was analyzed on the basis of GeneBank and GeneCards data . Results Seventeen DNA repair associated genes were identified and they were differentially expressed in various stages of gliomas, which were up regulated or down regulated during the malignant progression of gliomas. Conclusion Some DNA repair genes may play an important role in the process of malignant progression and ressistance of radiotherapy and chemotherapy against gliomas.
出处
《中华神经外科疾病研究杂志》
CAS
2004年第5期389-392,共4页
Chinese Journal of Neurosurgical Disease Research
基金
国家自然科学基金资助项目 (30 0 70 772 )
江苏省高校自然科学基金资助项目 (OIKJB32 0 0 0 2 )
