摘要
目的探讨胡麻油烟冷凝物 (SVC)对小鼠脂质过氧化及致突变作用。方法选择健康昆明种小鼠60只 ,随机分为6个剂量组 :SVC高剂量组 (10ml/kg)、中剂量组 (5ml/kg)、低剂量组 (2.5ml/kg) ,熟胡麻油对照组 ,阴性对照组(自来水 )和阳性对照组 (环磷酰胺50ml/kg) ,每组10只。除环磷酰胺腹腔注射3次外 ,其余5个剂量组均每日灌胃1次 ,连续15d ,测定血浆丙二醛 (MDA)含量及超氧化物歧化酶 (SOD)活性 ,并观察骨髓微核及精子畸形。结果胡麻油烟冷凝物10、5、2.5ml/mg剂量组及单纯胡麻油组小鼠血浆MDA含量均高于阴性对照组 (P<0.01) ,SOD活性明显降低 (P<0.01) ,各剂量组微核率、精子畸形率明显高于单纯胡麻油及阴性对照组 (P<0.01)。结论胡麻油烹调油烟冷凝物可引起小鼠脂质过氧化损伤和具有致突变作用。
Objective To study the effects of siritch vapor condensate (SVC) on lipid peroxidation of mice and its mutagenicity to mice. Methods 60 healthy Kunming mice were selected and randomly divided into 6 dose groups, 10 mice per group: SVC high_dose group (10 ml/kg), SVC middle_dose group (5 ml/kg),SVC low_dose group (2.5 ml/kg), prepared siritch control group, negative control group (tap water) and positive control group (cyclophosphamide, 50 ml/kg). Except the positive control group treated by peritoneal injection with cyclophosphamide 50 ml/kg for 3 times, the other 5 groups were treated by oral perfusion with different doses of SVC, prepared siritch and tap water once a day for 15 days. After exposure, the contents of malonyldialdehyde (MDA) and the activities of superoxidide dismutase (SOD) in plasma were determined, the frequency of micronuclei in bone marrow cells and counts of abnormal morphologic sperm were observed and analyzed in mice. Results The significantly higher contents of MDA and lower activities of SOD were observed in SVC 10 ml/kg, 5 ml/kg, 2.5 ml/kg and prepared siritch control groups compared with those in negative control group respectively (P<0.01). The frequencies of micronuclei in bone marrow cells and counts of abnormal morphologic sperm revealed higher levels in each SVC group and positive control group compared with those in siritch control group and negative control group (P<0.01). Conclusion SVC could induce the damages of peroxidation and mutagenicity to mice.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2002年第2期105-107,共3页
Journal of Environment and Health
