摘要
对小麦试管苗的管理,染色体加倍与移栽的研究结果表明,N_6+IAA0.2mg/L+Ad40mg/升+蔗糖2%的琼脂培养基壮苗促根效果较好,40天后平均每株试管苗可产生新根2.65条;通过调节光照,控制主茎叶龄数5—6,幼穗分化在伸长一单棱期,可以获得发育健壮的越夏试管苗。试管苗移栽前经简化营养液浸根6—7天。平均每苗可生新根1.16条,无根苗的生根率为41.6%;营养纸钵育苗20—25天后再连同纸钵移栽大田,成活率96.88%。试管苗的染色体加倍在冬,春季均可进行,1月5日至2月5日处理效果最好;用含营养成分的秋水仙碱溶液(简化营养液+0.05%秋水仙碱+1.5%DMSO,pH7)浸根,试管苗的成活率为88.10%,植株加倍率为52.70%。
N_6 agar medium with 0.2mg IAA, 40mg Ad and 20g sucrose perliter has good effect on promoting sturdy growth and development oftube shoots and their roots, after 40 days, per tube shoot can develop2.65 new roots. The oversummer sturdy tube shoots can be obtainedboth in controlling the main stems with 6-7 leaves and in the youngspike differentiation from the elongating to the single-rowed periodthrough regulating light. Befoer transplanting. the tube shoots soaked into the simple nutrient solution for 6-7 days, thus, per tube shootcan produce 1.16 new roots and the root-producing rate of the rootlesstube shoots is 41.67%. After the shoots are cultured in the nutrientpaper pots for 20-25 days, they are transplanted into the field withnutrient paper pots, the survived rate is 96.88%. The chromosomedoubling of tube shoots can be carried out in winter and spring, thebest time is from Jan. 15 to Feb. 15. The roots of tube shoots soakedinto colchicine solution containing nutrient (the simple nutrient solu-tion +0.05% of colchicine+1.5% of DMSO, with pH7), the survived rateand the doubling rate of plants are 88.10% and 52.70% respectively.
出处
《西北植物学报》
CAS
CSCD
北大核心
1991年第3期185-193,共9页
Acta Botanica Boreali-Occidentalia Sinica
关键词
小麦
试管苗
染色体加倍
栽培
Tube shoot of wheat
Leaf age of main stem
Young spike differentiation
Chromosome doubling
