摘要
为转移野生种的有利性状,进行了小麦体细胞不对称杂交的研究。由小麦品种"京花1号"和多花黑麦草的悬浮系分离原生质体通过电融合得到了大量的再生愈伤组织,并分化出了99株绿苗和大量白化苗。同工酶检测表明,所得到的植株带有多花黑麦草的部分遗传性状,是真杂种。为抑制亲本原生质体的分裂,分离原生质体前,多花黑麦草的悬浮细胞用软X-射线照射,以杀死部分细胞核;小麦的原生质体用2～4mmol/L的IOA室温暗处理15min。研究表明,受体悬浮系的再生能力愈强,融合的愈伤组织的再生能力也愈强,白化苗的再生频率也相对较低。
In order to transfer desirable traits from wild species, asymmetric somatic hybridization was carried out between wheat (Triticum aestivum L.) and Italian ryegrass (Lolium multiflorum Lam.). Protoplsats from suspensions of wheat cv. Jinhua No. 1 and Italian ryegrass were fund by Shimadzu SSH-2 somatic hybridizer, a large number of calli were derived, 99 green plants and many albino plants were regenerated. Isozyme analysis showed that some regenerated plants were true somatic hybrids by appearance of traits of Italian ryegrass. In order to inhibit the growth of parental protoplasts, the suspensions of Italian ryegrass were irradiated by soft X-ray before isolating protoplasts, while protoplasts of wheat were treated 15 min by IOA in the dark at room temperature at concentrations of 2～4 mmol/L. It is shown that the more the plant regenerability of recipient suspension was, the higher the plant regeneration frequency of calli derived from protoplast fusion was, and the lower the albino plant ratio was.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1997年第2期105-111,T001,共8页
Journal of Huazhong Agricultural University
关键词
杂交
小麦
黑麦草
植株再生
同功酶
asymmetric somatic hybridization, wheat (T. aestivum L. ), Italian rye grass (L. multiflorum Lam.), plant regeneration, isozyme
