摘要
目的 调查戊型肝炎病毒 (HEV)对猪的感染情况。方法 用HEV抗体试剂盒检测猪血清中的抗体 ;用逆转录聚合酶链方法 (RT PCR)检测猪血清中的HEVRNA ,对PCR阳性产物进行克隆测序 ,并对序列进行分析。结果 10份猪血清中有 1份为HEV抗体阳性 ,2份为HEVRNA阳性 ,其中 1份为抗体和RNA均阳性。序列分析显示 ,从猪中克隆的 2株序列 (G6和G8)之间在ORF1(10 2～ 387bp)和ORF2 (6 0 0 7～ 6 35 4bp)区域的核苷酸序列的同源性分别为 94 %和 93% ,该 2株序列在ORF1区与HEVⅠ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ和Ⅷ型的同源性分别为 76 %～ 79%、80 %、79%～ 80 %、88%～ 90 %、75 %～76 %、79%、78%～ 79%和 75 %～ 78% ;在ORF2区与Ⅰ、Ⅱ、Ⅲ和Ⅳ型的同源性为 75 %～ 77%、74 %～77%、77%～ 78%和 84 %～ 99% ,与ⅣA亚型的同源性为 93%～ 97%。
Objective To investigate HEV infection in hogs. Methods The HEV antibody was screened in sera collected from hogs with anti HEV EIA. HEV RNA was detected using RT PCR with ORF1 adn ORF2 primers. The positive PCR products were cloned and sequenced and compared with the identified HEV isolates. Results 2 of 10 samples were positive for HEV RNA and 1 of 2 samples positive for HVE RNA was also positive for anti HEV. The remaining 9 samples were negative for anti HEV. 2 sequences (G6, G8) were 94% and 93% identical to each other in ORF1 (102 387bp) and ORF2 (6 007 6 254bp) regions. They showed 76% 79%, 80%, 79% 80%, 88% 90%, 75% 76%, 79%, 78 79% and 75% 78% homogenicity to genotypes Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ, Ⅶ and Ⅷ in ORF1 region and showed 75% 77%, 74% 77%, 77% 78% and 84% 99% homogenicity to genotypes Ⅰ, Ⅱ, Ⅲ and Ⅳ in ORF2 region, especially had a 93% 99% identity to HEV subgenotype ⅣA in ORF2 region at the nucleotide acids. Conclusion HEV sequences isolated from hogs had a highest homogenicity to HEV ⅣA isolated from patients with acute hepatitis. [
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2002年第3期252-265,共14页
Chinese Journal of Microbiology and Immunology
