摘要
为了解某农村单采浆供血员人群所感染丙型肝炎病毒（ＨＣＶ）的血清型和基因型构成，并对两种分型方法进行比较，本工作以ＨＣＶＣ区型特异性多肽对抗－ＨＣＶ进行血清学分型，对已确定血清型的血清进行５′非编码区（ＮＣＲ）逆转录套式聚合酶链反应（ＲＴ－ｎＰＣＲ）和限制性片段长度多态性（ＲＦＬＰ）分析以确定基因型，并对６份扩增产物进行序列测定。结果显示１４０份抗－ＨＣＶ阳性血清中，血清Ⅰ型和Ⅱ型分别为４４份（３１．４３％）和１２份（８．５７％），余８４份未能分型。４４株已知血清型的ＨＣＶ中，１ｂ、２ａ和３ｂ等３种基因型分别为３４株（７７．２７％）、９株（２０．４５％）和１株（２．２７％）。两种分型方法一致率为９３．１８％（４１／４４）。对６株ＨＣＶ５′ＮＣＲ的序列分析证实了ＲＦＬＰ分型的正确性。结论认为该人群ＨＣＶ感染以血清Ⅰ型或基因１ｂ型为主；Ｃ区型特异性多肽血清学分型法与ＲＦＬＰ基因分型法符合率高，具有一定应用价值。
To investigate the prevalence of HCV serotypes and genotypes in a rural plasma donor population and to assess the two typing methods,serotype of anti HCV positive sera was identified by enzyme linked immunosorbent assays (ELISA) constructed with type specific synthetic peptides of the HCV core region,genotype was determined by restriction fragment length polymorphism (RFLP) analysis against partial 5′noncoding region(NCR) ( 274￣ 35).Of 140 anti HCV positive sera detected,44(31 43%) were serotype Ⅰ,12(8 57%) were serotype Ⅱ,and the rest undetermined.In 44 HCV RNA positive sera which serotypes had been determined,subtype 1b,2a and 3b amount to 34(77 27%),9(20 45%) and 1(2 27%).The consistency between type specific core peptide ELISA and 5′NCR RFLP analysis was 93 18%(41/44).Sequence analysis for 6 isolates of HCV coincided with the result of 5′NCR RFLP.The most prevalent subtype of HCV was 1b in this population;Being well consistent with the genotyping method,type specific core peptide ELISA is thought to be a useful tool for HCV typing.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1997年第2期88-91,共4页
Chinese Journal of Microbiology and Immunology
