摘要
目的 探讨痢疾菌苗滴鼻免疫后，小鼠不同粘膜部位和其它部位免疫应答的影响。方法将Ｂａｌｂ／ｃ小鼠随机分为３组，每组１５只。舰疾菌苗株 ＴＩＭ－２１１７或 ＦＳ－５４１６，按 ５×10～6、１×１０～７、４×１０～７和４×１０～７ＣＦＵ／只滴鼻跟 Ｂａｌｂ／ｃ小鼠４次涧隔两 ｗｂ。分离鼻相关淋巴组织（ＮＡＬＴ）、鼻通道（ＮＰ）及脾、小肠的ＰＰ结淋巴细胞。一部分细胞采用流式细胞术检测淋巴细胞表型的变化；另一部分细胞与全菌破碎抗原共培养，于不同时间点取出，提取ＲＮＡ做ＲＴ－ＰＣＲ。结果两株菌苗经鼻内免疫后，ＮＡＬ、ＮＰ和ＰＰ结的淋巴细胞中ＣＤ３Ｔ细胞显著增加，其中以ＣＤ４＋Ｔ细胞增加为主。ＴＳＭ－２１１７株免疫组的脾细胞中，Ｂ２２０＋细胞显著增加；而ＦＳ－５４１６株免疫组的脾细胞中，ＣＤ３＋Ｔ细胞显著增加。免疫小鼠的ＮＡＬＴ、ＮＰ和ＰＰ结淋巴细胞，在体外经抗原刺激后，均在不同时间出现ＩＦＮ－γ和ＩＬ-４ｍＲＮＡ的表达，ＴＧＦ－β ｍＲＮＡ的表达在免疫前后无明显变化。结论痢疾菌苗经鼻粘膜免疫，可诱导不同粘膜部位及全身免疫应答的产生。
Aim To observe the effect on immune responses at dif- ferent mucosal and other sites of body after intranasal immunization with bivalent shigella vaccines. Methods Balb/c mice were di- vided into three groups at random , 15 mice per group. Mice were intranasally immunized respectively with FSM-21 l7or FS-5416 of four doses (5×10~ 6 ,1×10~7,4×1O^7 and 4 ×1O^7CFU) at an interval of two weeks . Lymphocytes were isolated from the nasal associated lymphoid tissue (NALT), nasal passage (NP), spleen, Peyer ‘s patches (PP), mesenteric lymphoid node (MLN), and then on the seventh day after the last immunization the changes of the eymoho- cytic cell phenotype were analyzed by FACS. Some of these eymo- hocytes were cells cocultured with smashed bacterial antigen for va- rious time, then RNA was extracted from these cells and RT-PCR was carried out. Result CD3 ^+ cells in NALT .NP and PP lym- phocytes increased significantly after immunizatron ,in which CD4~ + T cells increased mainly. B220^+ cells in splenocytes increased in FSM-2l17 group and CD3 + T cells in FS-5416 group. After restim- ulated with the antigen, the lymphocytes from NALT, NP and PP all expressed IFN-γ and IL-4 mRNA at various time, but the ex- pression of TGF-β mRNA daid not differ signiticantey between the nonimmunized and immunized groups. Conclusion Intranasal in- oculation of shigella vaccine can induce specific immune response in various mucosaal sites and other sites of body
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第1期49-51,共3页
Chinese Journal of Cellular and Molecular Immunology
