摘要
利用PCR技术从原始菌种中扩增得到K99和LTB基因,将K99和LTB基因连接起来,构建了LTB-K99融合基因。融合基因克隆到pBS-T载体上,转化大肠杆菌top10,经Bam HI和Xho I双酶切鉴定重组质粒。测序分析结果表明,该融合基因有正确的阅读框,为以后转入表达载体的构建奠定了基础。
The 5＇ terminus of heat-labile was fused to the 3＇ terminus of k99 Fimbrial antigen gene by PCR. The LTB-k99 fusion gene was cloned into pBS-T vector, transfer red into E. coli top10,and sequenced by using universal primers. The gene sequence determination showed the correct .sequence and reading frame.
出处
《石河子大学学报（自然科学版）》
CAS
2006年第1期98-101,共4页
Journal of Shihezi University(Natural Science)
关键词
肠毒素大肠杆菌
LTB基因
K99基因
融合基因
Enterotoxigenic escherichia coli（ETEC）
heat-labile toxin
k99 fimbrial antigen
fusion gene
