摘要
通过Ｐｌｋｃ噬菌体将Ｔｎ１０插入灭活的ａｒｏＤ基因片段导入双价有毒痢疾菌ＦＳ－１５中，构建了该株的芳香族氨基酸营养缺陷减毒株，并通过Ｂｏｃｈｎｅｒ培养基去除Ｔｎ１０所携带的四环素抗性，经原位杂交从中检测到ａｒｏＤ基因缺失株ＦＳ－５４４１。该株稳定表达福氏及宋内氏双价Ｏ抗原，Ｗｅｓｔｅｒｎ－ｂｌｏｔ显示该株表达Ｉｐａ蛋白，具有穿入ＨｅＬａ细胞的能力，穿入率为１９％。营养缺陷表型的回复突变率小于１０￣（－１１）。小白鼠毒力试验证明安全无毒，免疫小白鼠后对福氏及宋内氏毒株的攻击具有保护活性。
In
this study,we constructed 10 attenuated strains by intruducing Tn10
inactived aroDgene into FS-15,a virulent bivalent hybrid strain of S.
flexneri 2a and S.sonnei constructed inour laboratory in 1988,and
then by using a modified bochner’s medium,a series of
tetracycline-sensitive mutants were obtained.One mutant of them
FS-5441 was demonstrated aroD genedeletion with a colony-hybridation
with specific aroD gene probe,FS-5441 was found to invadeHela cells
in vitro and negative in Sereny test.It expressed stably the somatic
antigen of S.flexneri 2a and S.sonnei and exhibited significant
protection for immunnized mice against thechallenge of S.flexeneri 2a
and S.sonnei virulent strains.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1995年第2期68-69,共2页
Chinese Journal of Immunology
