摘要
目的 探讨缺氧诱导因子（ＨＩＦ）在脑弥漫性轴索损伤中表达的意义。方法利用大鼠头颅瞬间侧向旋转模型造成弥漫性脑损伤，采用免疫组化法测定 ＨＩＦ－ｌα的表达，并用ＴＵＮＥＬ和免疫组化双重标记法，以及双酶免疫组化法，研究ＨＩＦ-1α表达与神经细胞凋亡的关系。结果弥漫性轴索损伤后，ＨＩＦ－ｌα在丘脑、海马和基底核区神经细胞中呈高表达，并可诱发ｃ-ｍｙｃ和Ｆａｓ基因表达，启动神经细胞凋亡。
Aim To study the significance of HIF expression in neu- ron of brain diffuse axonal injury( DAT). Methods The brain injury model of 20 adult rats DAI was created by instant head lateral rota- tion. Expression of HIF-lain DAI specimen was detected by immu- nohistochemical ( IHC) staining. The relationship between neuron apoptosis and HIF- 1α expression was investigated by double label- ling technique of TUNEL and IHC staining, and IHC staining with two enzymes. Results The level of HIF-lα expression was high in neurons of thalamus, hippocampus and basal ganglia, accompanied by expression of c-myc and Fas genes which triggered apoptosis of neurons. Conclusion HIF- 1 α may be one of the important factors that induace neuron apoptosis after brain DAI.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第1期33-35,共3页
Chinese Journal of Cellular and Molecular Immunology
