摘要
目的探讨胸腺基质细胞（ＴＳＣ）对胸腺细胞的调节作用。方法应用光镜、电镜和流式细胞术等，观察初代培养的ＴＳＣ对胸腺细胞发育的调节作用。结果光镜和电镜均可见，ＴＳＣ可大量粘附和吞噬胸腺细胞，其中部分胸腺细胞发生凋亡。尽管与ＴＳＣ共培养的胸腺细胞存活率高，凋亡率低，但其总数却显著减少。结论ＴＳＣ可通过粘附和吞噬作用，清除部分胸腺细胞。
Aim To explore the regulatory effect of thymic stronial cells ( TSC) on thymocytes. Methods Light microscopy, electron microscopy and flow cytometry ( FCM) were used to observe the reg- ulatory effect of cultured primary TSCs on thymocytic development. Results A large number of thymocytes adhered to and were en- gulfed by TSCs. Some engulfed thymocytes showed apoptosis. Al- though thymocytes cocultured with TSCs had high survival rate and low apoptotic rate, their total number decreased significantly. Con- clusion ThCs can delete some thymocytes via their adhesion and phagocytosis.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第1期20-22,共3页
Chinese Journal of Cellular and Molecular Immunology
关键词
胸腺基质细胞
胸腺细胞
凋亡
调节
thymic stromal cells
thymocytes
apoptosis
regula-tion
