摘要
目的 探讨内毒素（ＬＰＳ）和地塞米松（Ｄｅｘ）对体外培养的大鼠肺泡巨噬细胞（ＡＭ）中ＮＦ－ＫＢ活化的影响，以及ＡＭ中ＮＦ－ＫＢ活化在急性肺损伤中的可能作用。方法 通过建立大鼠ＡＭ体外培养技术，应用免疫组化染色法，观察ＬＰＳ和Ｄｅｘ寸 ＡＭ中 ＮＫ－ＫＢＰ～（６５），ＩＫＢ－α、ＴＮＦ－α和 ＩＣＡＭ－ｌ蛋白表达的动态变化。结果 ＬＰＳ能使培养的ＡＭ中 ＮＦ－ＫＢＰ～（６５），ＴＮＦ－α和ＩＣＡＭ－ｌ的表达增加，ＩＫＢ－α的表达降低；Ｄｅｘ的作用与 ＬＰＳ恰相反。结论ＬＰＳ促进ＡＭ中的ＮＦ－ＫＢ活化，可能是急性肺损伤肺内炎症损害发生的启动及促进因素；Ｄｅｘ抑制 ＮＦ－ＫＢ的活化，可能是其抗炎作用的重要机制之一。
Aim To explore the effects of LPS and Dex on NF- KB activation of cultured alveolar macrophages ( AMs) and to study the role of AMs in the pathogenesis of acute lung injury. Methods AMs were harvested by bronchoalveolar lavage technique ,and cul- tured in vitros. The influences of LPS and Dex on expression of NF- KB^(65) ,IKB- α、TNF- α and ICAM- 1 proteins in the AMs were evalu- ated at the different time points ( 2,6, 12 and 24 hours after cul- ture) by immunohistochemical staining. Results LPS could in- crease the expression of NF- KB P^(65),TNF- α、and ICAM- 1 proteins and decrease 1KB- α in the AMs. The influences of Dex on the ex- pression of these proteins were contrary to those of LPS. Conclu sion LPS can promote NF- KB activation in AMs , which may contribute to the pathogenesis of acute lung injury. Dex can inhibit NF- KB activation in AMs ,which may be a mechanism of anti - in- flammation by Dex.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2002年第1期13-15,共3页
Chinese Journal of Cellular and Molecular Immunology
关键词
肺泡巨噬细胞
地塞米松
内毒素
NF-κB
细胞间粘附分子1
TNF-α
IΚB-Α
alveolar macrophages
lipopolysaccharide
dexanietha-sone
nuclear factor kappa B
intercellular adhesion molecule - 1
tumor necrosis factor- α
IKB- α.
