摘要
目的:研究呼吸道合胞病毒(RSV)感染与细胞凋亡的关系及对凋亡相关基因FasL、Fas、bcl-2和bax表达的影响。方法:采用A549细胞,在RSV感染后不同时点收集细胞,流式细胞仪和透射电镜检测细胞凋亡,免疫组织化学法检测凋亡相关基因FasL、Fas、Bcl-2和Bax表达情况。结果:流式细胞仪检测结果RSV感染后72h(6.61%)、120h(10.94%)的细胞凋亡指数明显高于对照组(4.32%、5.31%);免疫组化法检测结果对照组FasL、Bax基因呈现无表达或局部弱表达;随着RSV感染时间的延长,bax、Fas、FasL基因的表达均高于对照细胞,bcl-2基因呈现弱表达或无表达。结论:RSV在感染后期能诱导宿主细胞发生凋亡,促凋亡基因FasL、Fas、Bax和抗凋亡基因Bcl-2表达水平的差异是RSV诱导凋亡的机制之一。
AIM： To study the relationship between respiratory syncytial virus （RSV） infection and apoptosis, between RSV infection and expressions of FasL, Fas, Bcl - 2 and Bax. METHODS： Apoptotic cells were examined by flow cytometry and transmission electron microscope, hnmunohistochemical analysis was used to detect the expressions of apoptosis - associated gene Fasl., Fas, Bcl- 2 and Bax in A549 cells during RSV infection. RESULTS： Apoptotic index increased at 72 h and 120 h postinfection. Apoptotic cells were deta：ted by transmission electron microscope. High - expressions of FasL, Fas and Bax genes and low -expression of Bcl- 2 gene were detected by immunohistcochemical staining. CONCLUSION： Apoptosis in A549 ceils was induced by RSV infection. This apoptosis may be induced by upregulating the expression of FasL, Fas, Bax genes and down - regulating the expression of Bcl - 2 gene.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第9期1748-1751,共4页
Chinese Journal of Pathophysiology
