摘要
目的 :观察大鼠脑缺血再灌注后不同时间缺血区细胞间粘附分子 1(ICAM 1)的表达和中性粒细胞的浸润以及神经细胞的损伤情况。方法 :40只 Wistar大鼠分为对照组、假手术组和缺血 2小时再灌注 2、4、12、2 4、48、96小时组 ,分别用免疫组织化学和组织切片方法检测大脑中动脉阻塞 2小时再灌注不同时间点局部脑组织 ICAM 1蛋白表达及中性粒细胞浸润情况。结果 :大鼠脑缺血再灌注 2小时局部脑组织 ICAM 1的表达开始升高 ,于再灌注 48小时达到峰值 ,持续至再灌注 96小时仍保持较高水平表达 ;局部缺血区域中性粒细胞浸润于再灌注后 12小时开始升高 ,48小时达到高峰 ,与 ICAM 1的表达呈同步改变。结论 :脑缺血再灌注后表达增高的 ICAM 1与局部缺血区中性粒细胞的浸润密切相关 。
Objective:To investigate the expression of intercellular adhesion molecule1(ICAM1) and neutrophil infiltration after cerebral ischemiareperfusion in rats.Methods:40 male Wistar rats were randomly divided into 3 groups:control group,sham operation group and cerebral ischemiareperfusion group (reperfusion for 2,4,12,24,48,96 hours postischemia).Immunohistochemical and histological techniques were respectively used to evaluate ICAM1 expression and neutrophil infiltration in focal brain tissue after various duration of reperfusion following middle cerebral artery occlusion(MCAO) for 2 hours in rats.Results:The expression of ICAM1 started to increase after reperfusion for 2 hours,peaked at 48 hours after reperfusion,and continued for 96 hours.Neutrophil infiltration in ischemic tissue increased at 12 hours,and peaked at 48 hours after reperfusion,paralleling the increase in ICAM1 expression.Conclusions:The increased expression of ICAM1 after cerebral ischemiareperfusion is significantly correlated with the degree of the neutrophil infiltration in focal ischemic brain tissue,which may be an important mechanism of ischemiareperfusion damage.
出处
《中国危重病急救医学》
CSCD
2000年第5期264-266,共3页
Chinese Critical Care Medicine
基金
国家自然科学基金资助项目!(39670268)
关键词
再灌注损伤
白细胞浸润
脑缺血
ICAM-1
粘附分子
cerebral ischemiare-perfusion injury
intercellular adhesion molecule-1
neutrophil infiltration
