摘要
本研究利用Tripure试剂盒 ,成功地提取了斯氏按蚊总RNA ,并对不同方法保存的标本所提RNA的质量进行了比较 ,结果显示新鲜标本所提RNA质量最高 ,将标本在Tripure提取液中匀浆后置于 4℃保存的方法 ,较其它两种方法保存效果更好 ,此方法保存的标本 5天内进行RNA提取 ,能够保证质量。
The total RNA of An. stephensi was successfully isolated with Tripure isolation reagent. The qualities of the total RNA isolated from mosquitoes preserved by different ways were compared. The results showed that the RNA isolated from fresh sample were the best. Soaking the sample in the Tripure isolation reagent at 4℃after the sample being homogenized was better than the two other ways and if the RNA isolated from the sample preserved within 5 days by this way, the quality would be ensured.
出处
《四川动物》
CSCD
2002年第2期67-68,F004,共3页
Sichuan Journal of Zoology
