摘要
应用异硫氰酸胍-酚-氯仿一步法抽提日本血吸虫成虫RNA,能够在数小时内抽提出完整的成虫RNA。此法对于分离数量很少的寄生虫材料中的RNA尤为有效。通过对日本血吸虫成虫RNA变性琼脂糖凝胶电泳分析,发现其核糖体大亚基RNA(L-rRNA)体内切口(in vivo nick)现象的存在。并随核糖体小亚基RNA(S-rRNA)一道电泳迁移。反映了L-rRNA的后转录加工。
A rapid method of total RNA of Schistosoma japonicum adult worm isolation by single step extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of intact RNA and can be completed within several hours. It is particularly useful for isolation of RNA from minute quantity of parasite material. Analysis on agarose gels electrophoresis reveals that the RNA preparations show a distinct band comigrating with 18S rat brain RNA and without large rRNA species. The large rRNA is in vivo nicked. The absence of a band at approximately 26S presumably reflects post transcriptional processing of the large rRNA.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1993年第2期135-137,共3页
Chinese Journal of Parasitology and Parasitic Diseases
