摘要
目的 :观察不同剂量氯胺酮 (KT)对腹腔感染小鼠死亡率和血浆肿瘤坏死因子 α(TNFα)水平的影响 ,并探讨其可能的机制。方法 :以盲肠结扎并穿孔 (CL P)制作腹腔感染模型。将小鼠随机分为假手术组、CL P组、KT(CL P加 KT)组、阴性对照 (CL P加戊巴比妥钠 )组。 KT组经肌肉注射不同剂量 KT,观察 1周内死亡情况 ;并采用酶联免疫吸附法测定血浆 TNFα含量。结果 :KT 5 0 m g/ kg、每日 3次时 ,小鼠死亡率最低 ,且注射KT能够降低 CL P小鼠血浆 TNFα含量。结论 :一定剂量的 KT可以降低腹腔感染小鼠的死亡率 ,并抑制血浆 TNFα浓度的增加 。
Objective:To investigate the effects of ketamine (KT) on mortality and plasma concentration of tumor necrosis factorα(TNFα) in septic mice.Methods:Cecal ligation and puncture (CLP) was used to make abdominal infection model.Healthy male mice were divided into Sham group,CLP group,KT (CLP+KT) group,and control (CLP+sodium pentobarbital) group.KT groups were treated with different doses of ketamine and the mortality rates were observed during a week.TNFα was assessed with enzymelinked immunosorbent assay(ELISA).Results:The mortality rate of CLP mice was the lowest when ketamine was given infused via muscle at 50 mg/kg three times per day.Plasma TNFα levels decreased as compared with CLP group.Conclusions:Ketamine would decrease the mortality rate and inhibit TNFα production in septic mice.
出处
《中国危重病急救医学》
CAS
CSCD
2002年第5期273-275,共3页
Chinese Critical Care Medicine
基金
国家自然科学基金资助项目 (No.3 9670 3 0 9)
关键词
氯胺酮
腹腔感染
死亡率
脓毒症
ketamine
abdominal infection
mortality
sepsis
