摘要
目的测定健康人血清中7种游离脂肪酸(FFA)含量。方法采用气相色谱法(GC)测定血清FFA。血清样品前处理:以正十七酸为内标,用硫酸/甲醇溶液对血清中FFA进行甲酯化处理。结果α亚麻酸、花生四烯酸、二十碳五烯酸和二十二碳六烯酸的线性范围在2～300μg/mL,硬脂酸、油酸和亚油酸的在50～1000μg/mL(r>0.999)。方法回收率良好(95.65%～104.88%)。各种脂肪酸的日内变异系数为0.36%～2.13%(n=5),日间变异系数为0.82%～3.36%(n=5)。结论气相色谱法测定血清FFA方法灵敏、准确,可用于临床。
Objective To establish method for the determination of 7 free fatty acids in human serum. Methods Free fatty acids were detected by gas chromatography（GC）. The extracted lipids were methylesterified by H2SO4/methanol. Heptadecanoic acid（C17 ： 0） was used as internal standard. Results The linearity of C18 ： 3, C20 ： 4, C20 ： 5 and C22 ： 6 ranged from 2-300 μg/mL, and linearity of C18 = 0, C18 = 1 and C18 ： 2 ranged from 50-1 000 μg/mL（r〉0. 999）. The recovery rate was 97. 65% - 104.88 %. The intra-day variation coefficient was less than 2. 13 % and 3. 36 %, respectively. Conclusion This method proved to be sensitive, reproducible, and applicable for the determination of free fatty acid in serum.
出处
《福建医科大学学报》
2006年第2期179-181,共3页
Journal of Fujian Medical University
基金
福建省自然科学基金资助项目(Z0516070)
关键词
脂肪酸类
甲酯化
色谱法
气相
血清
fatty acids, methylesterified
gas chromatography
serum
