摘要
目的 :探讨血小板膜糖蛋白 (GP)Ⅰa基因 80 7C/T多态性在不稳定性心绞痛 (UAP)发病机制中的作用。方法 :①体外应用Ⅰ型胶原诱导 ,观察 35例UAP(UAP组 )患者和 33例正常人 (正常对照组 )血小板聚集功能的变化 ;②用酶联免疫双抗体夹心法测定上述人群中血浆α颗粒膜蛋白 (GMP) 14 0浓度 ;③应用聚合酶联扩增实验 (PCR SSP)对上述人群进行血小板膜GPⅠa基因 80 7C/T多态性检测。结果 :①UAP患者和正常人TC基因型体外胶原诱导下血小板达 30 %聚集率前的迟缓期较CC基因型均显著缩短(P <0 0 5 ) ,但二者的最大血小板聚集率无显著性差异。UAP组与正常对照组对比 ,同基因型相比血小板达 30 %聚集前的迟缓期明显缩短 (P <0 0 1) ,最大血小板聚集率无显著差异。②UAP患者TC基因型血浆GMP 14 0浓度明显高于CC基因型 (P <0 0 5 ) ,与正常对照组同基因型比较亦有显著差异 (P <0 0 1)。③UAP组GPⅠa基因 80 7C/T中TC基因型比例 ( 5 7 14 % )大于正常对照组 ,但统计学无显著性差异。结论 :与血小板膜GPⅠa基因T80 7等位基因相关的胶原诱导下血小板聚集功能的迅速启动 ,可能为UAP发病的机制之一。
Objective: To assess the role of platelet membrane glycoprotein (GP)Ⅰa gene polymorphism in the pathogenesis of unstable angina pectoris(UAP). Methods: ①Collagen type Ⅰ-induced platelet aggregation was measured in vitro in 35 patients with UAP and 33 normal controls. ②Plasma level of α-granule membrane protein (GMP-140) was measured. ③Samely, the GP Ⅰa gene 807C/T polymorphisms were examined by polymerase chain reaction-sequence specific primers (PCR-SSP). Results: ①The lag time before 30% platelet aggregation was significantly shorter in the subjects (UAP patients and controls) with TC genotype than in those with CC genotype (p<0.05), but no significant difference was found in the maximal platelet aggregation between the two genotypes. Between the UAP group and normal control group, the lag time before 30% platelet aggregation was significantly difference. ②In the UAP group the plasma level of GMP-140 was significantly higher in patients with TC genotype than in those with CC genotype (p<0.05). There was also a difference between UAP group and control group (p<0.01).③The TC genetype of GPⅠa gene was higher in the UAP group than in the control group,but there was no stastically significance. Conclusion: The rapid initiation of collagen-induced platelet aggregation is associated with GP Ⅰa gene T 807 allele, which may play an important role in the pathogenesis of UAP.
出处
《中国循环杂志》
CSCD
北大核心
2002年第2期124-126,共3页
Chinese Circulation Journal
