摘要
目的测定川崎病(KD)患儿血浆纤溶酶原激活物抑制剂-1(PAI-1)、血小板α-颗粒膜蛋白(GMP-140)及血浆内皮素(ET)的水平,研究纤溶活性降低、内皮细胞损伤和血小板活化在KD及冠状动脉损伤中的临床意义。方法用酶联免疫双抗体夹心法分别测定35例KD患儿PAI-1、GMP-140浓度,用放射免疫分析法测定血浆内皮素(ET),并与35名健康对照组儿童的结果进行比较。结果KD患儿组的PAI-1急性期、恢复期均高于健康对照组,差异有统计学意义(P<0.01),而且恢复期持续增高。GMP-140及ET急性期高于健康对照组,差异有统计学意义(P<0.01)。合并冠状动脉病变(CAL)组PAI-1、GMP-140、ET明显高于非合并冠状动脉病变(NCAL)组,差异有统计学意义(P<0.01)。且CAL组患儿PAI-1与GMP-140均与ET呈显著正相关(r=0.699,P<0.01;r=0.64,P<0.05)。结论内皮细胞损伤、纤溶活性降低和血小板的活化与KD冠状动脉血管损伤的发生、发展有着密切的关系。PAI-1、GMP-140和ET可作为预测和观察KD合并冠状动脉损伤病情的指标。
Objective To understand the roles of low fibrinolytic activity, platelet activation and endothelial injury in Kawasaki disease （KD） by determination of plasminogen activator inhibitor-1 （PAI-1）, platelet alpha granule membrane protein-140 （GMP-140） and endothelin （ET） in plasma of patients with this condition. Methods Plasma levels of PAI-1 and GMP-140 were measured by double-antibody sandwich enzyme-linked immunosorbent assay and plasma level of ET detected by radioimmunoassay among 35 KD patients and 35 healthy children. Results Compared with the healthy children, plasma level of PAI-1 was significantly higher in both acute phase and recovery groups （P 〈 0.01 ）, and remained at a persistently high level during convalescence. Plasma GMP-140 and ET in the acute phase group were significantly higher than those in the healthy group （P〈 0.01 ）. Levels of PAI-1, GMP-140 and ET in CAL group were significantly higher than in NCAL group （P 〈 0.01 ）. PAI-1 and GMP-140 in CAL group positively correlated with ET （r=0.699, P〈0.01; r=0.64, P〈0.05）. Conclusion Low fibrinolytic activity, platelet activation and endothelial injury are closely related to the development of coronary artery lesion in KD. PAI-1 ,GMP-140 and ET may be indicators for diagnosis and prognosis of coronary artery lesion in KD.
出处
《中国药物与临床》
CAS
2006年第12期905-908,共4页
Chinese Remedies & Clinics
关键词
黏膜皮肤淋巴结综合征
血小板活化
纤溶活性
内皮损伤
冠状动脉损害
Mucocutaneous lymph node syndrome
Platelet activation
Fibrinolytic activity
Endothelial injury
Coronary artery lesion
