摘要
目的：表达重组FS-288，制备抗FS抗体，建立特异性的FS双抗体夹心ELISA检测系统。方法：采用表达质粒构建及诱导表达方法制备重组FS-288，杂交瘤技术制备抗FS单抗，酶联免疫方法测定血中FS水平。结果：以抗FS多克隆抗体和单克隆抗体建立了 FS双抗体夹心ELISA法，其敏感度为25 pg/well，正常女性血中 FS水平随月经周期呈周期性变化，在排卵前2 d达峰值，而 acivin A无明显的周期性变化。结论：FS水平随月经周期改变，提示FS可能与性腺轴的内分泌调控有关。
Abstract Objective:To express recombinant follistatin 288(FS-288) and establish a specific two-site enzyme-linked immunosorbent as-say(ELISA) for native follistatin. Methods:The recombinant GST-FS-288 was expressed by using constructed expressive plasmid and inducingby IPTG. The anti-FS monoclonal antibody was prepared using a hybridoma technique. The FS levels in sera were determined by ELISA.Re-sults:The anti-FS monoclonal and polyclonal antibody was prepared by immunizing as antigen using the recombinant FS and purified by usingprotein A affinity chromatography. The sensitivity of the two-site ELISA for native follistatin established by anti-FS monocled and polyclonalantibody was 25 pg/well. Follistatin level in blood of normal women cchanged periodically during the menstrual cycle, its peak was two dsys be-fore ovulation, to contrast activin A level did not markedly changed during the menstrual cycle. Conclusion:The changes of follistatin level inthe serum of normal women during the menstrual cycle suggest the follistatin play an endocrine role in modulating gouadal activities.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2002年第5期348-352,共5页
Chinese Journal of Immunology
基金
吉林省科技发展计划项目(980580)
卫生部优秀青年科技人才专项科研基金(97049)
