摘要
检测 Mdrl基因表达水平可预测白血病化疗效果 ,用原位杂交的方法可检测 Mdrl在单个细胞的表达水平。本文用Rt- PCR的方法获得了一段特异的 c DNA片段 ,将其克隆到 PGEM4Z载体中 ,经 DNA序列分析证明与文献报道一致。采用地高辛素 (DIG) RNA标记试剂盒制备反义 RNA探针 。
The chemotherapy effects of patients with leukemia are correlated with the expression level of Mdrl gene which can be measured by ISH in individual leukemic cells because of the cDNA cloning of the human Mdrl. A special part of Mdrl cDNA was obtained by Rt PCR method and inserted into vector PGEM4Z. The DNA sequencing result showed it was identical with the sequence of Mdrl cDNA reported. The Mdrl RNA probe was made from the cDNA by use of DIG RNA labeling kit. The detecting system of Anti Dig AP was used on leukemia marrow smears and showed high specificity and sensibility.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2000年第1期86-88,共3页
Chinese Journal of Histochemistry and Cytochemistry
