摘要
目的：将简便可靠的非同位素原位杂交方法应用于急性白血病多药耐药基因（Ｍｄｒ１）的临床检测。方法：自行构建ＲＮＡ探针，经地高辛标记，直接在骨髓涂片上进行原位杂交，检测４５ 例急性白血病骨髓细胞Ｍｄｒ１ ｍ ＲＮＡ。对部分Ｍｄｒ１ 高表达者调整临床治疗。结果：杂交信号清晰，重现性好。复治组中Ｍｄｒ１ 阳性８８．２％ ，初治组４２．８％ ，差异有极显著性。经标准化疗２ 疗程后初治组中Ｍｄｒ１ 阳性组完全缓解（ＣＲ）率２２．２％ ，阴性组６３．６％ （ Ｐ＜ ０．００１）。对Ｍｄｒ１ 阳性病例调整化疗方案或加用逆转剂可改善预后。结论：Ｍｄｒ１ 高表达确为白血病不良预后的主要指标。本方法操作简便，结果可靠，可用于急性白血病Ｍｄｒ１ 常规检测，辅助临床治疗并及断预后。
Objective:To detect the Mdr1 gene in acute leuvemia .Method::Cytospins in situ hybridization(ISH) were performed with Dig nick translated RNA probes.Result:The positive rate of Mdr1 expression in relapse and refractory patients was much more higher than that in initial cases(P< 0.001 ).Complete remission rate was obviously lower in overexpression cases of the initial acute leukemia group.Better prognosis was obtained through modulation of remission inducer regimens or addition of reversal agents.Conclusion:Overexpression of Mdr1 mRNA is truly one of the important indication of poor prognosis in acute leukemias and the ISH method could be used satisfactorily in predicating clinical drug resistance and directing the rearrangement of chemotherapy regimens in view of its sensitivity and simple procedure.
出处
《临床血液学杂志》
CAS
1999年第4期146-149,共4页
Journal of Clinical Hematology
