摘要
将里氏木霉总RNA反转录得到cDNA第一链,并以之为模板进行 RT-PCR合成约1.4kb的纤维二糖酶基因cDNA,将所得的cDNA经测序后克隆到温度敏感型表达载体pJW2中,经PCR和双酶切鉴定筛出阳性重组子,温度诱导后,经酶活测定,bgl II基因在大肠杆菌中得到了表达,酶活为0.75IU/mL。
Cellobiase is a hydrolytic enzyme which acts on various compounds with b-D-glucosidic linkages. It plays a key role in hydrolyzing cellobiose to glucose. Cellobiase bglII gene was cloned by RT-PCR using first strand cDNA as template after reverse transcripting T.reesei total RNA. After sequenced, the bglII cDNA was then inserted directionally into a temperature-inducible prokaryotic expression vector pJW2. The bglII gene was expressed in E.coli DH5a.
出处
《菌物系统》
CSCD
北大核心
2002年第2期223-227,共5页
Mycosystema
基金
浙江省科委国际合作项目
