摘要
采用cross-overPCR方法将里氏木霉β-葡萄糖苷酶基因的外显子进行体外拼接,成功获得了剔除两个内含子的β-葡萄糖苷酶基因bgl1;进一步构建了重组质粒pYX-tbgl,并在酿酒酵母SaccharomycescerevisiaeW303-1A中获得表达,得到的转化子能以纤维二糖为唯一碳源生长。
The bgl1 gene encoding β-glucosidase Trichoderma reesei was amplified successfully by cross-over PCR method, and two introns were deleted. Therefore, a recombined plasmid pYX-tbgl inserted with bgl1 gene was constructed and expressed in Sac charomyces cerevisiae W303-1A. The recombinants grew well on cellubiose as a sole carbon source.
出处
《酿酒科技》
北大核心
2006年第9期17-20,共4页
Liquor-Making Science & Technology
基金
国家科技部"十五"攻关项目(2001BA501A01)资助。
关键词
微生物
里氏木霉
基因克隆
酿酒酵母
microbe
Trichoderma reesei
gene cloning
Saccharomyces cerevisiae
