摘要
研究了传染性法氏囊病病毒（IBDV）变异E株人工感染28日龄SPF雏鸡后鸡法氏囊淋巴细胞的凋亡情况。电镜观察和DNA电泳分析结果表明,IBDV感染后12～48h,雏鸡法氏囊淋巴细胞出现典型细胞凋亡的形态学特征和生化特征;经流式细胞计检测和荧光染色观察,统计学分析表明,IBDV感染后24～48h,雏鸡法氏囊淋巴细胞凋亡数量显著增加（P<0.05或P<0.01）。试验结果揭示IBDV变异E株人工感染可以诱导雏鸡法氏囊淋巴细胞凋亡。
Apoptosis of the bursal lymphocytes of chickens infected with variant serotype-1 isolate E of infectious bursal disease virus(IBDV) at 28-day-old was studied. The IBDV-infected bursal lymphocytes exhibited the morphological and biochemical features of apoptosis at 12 -48 h after infection using transimission electron microscope and agarose gel electrophoresis of cellular DNA. Statistical analyses by flow cytometry and staining with fluorescent DNA-binding dyes indicated that the number of apoptotic cells in the bursal lymphocytes increased signficantly by 24-48 h post infection(P<0.05 or 0.01). The results revealed that the bursal lymphocytes of chickens infected artifically with IBDV was induced to display apoptosis.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2002年第2期180-184,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
传染性法氏囊病病毒
变异E株
细胞凋亡
SPF鸡
免疫抑制
Variant serotype-1 isolate E of infectious bursal disease virus
Apoptosis Bursa of Fabricius
SPF chicken
