摘要
目的 探讨实验性自身免疫性脑脊髓炎 (EAE)大鼠脑血管内皮细胞表面分子的改变。 方法 用免疫组织化学方法检测EAE大鼠脑血管内皮细胞上血管细胞粘附分子 1(VCAM 1)和主要组织相容性复合体 (MHC)Ⅱ类分子的表达。用MTT(四甲基偶氮唑盐 )法检测不同发病级别大鼠血清TNF和IFN γ的水平。 结果 EAE大鼠脑血管内皮细胞较对照有诱导表达的VCAM 1和MHCⅡ类分子 ,血清中存在TNF和IFN γ活性。 结论 中枢神经系统炎症状态下 ,脑血管内皮细胞表面分子发生改变 。
Objective To explore the surface molecule changes of the experimental autoimmune encephalomyelitis (EAE) rat brain vascular endothelial cells. Methods The expressions of vascular cell adhesion molecule\|1(VCAM\|1) and major histocompatibility complex (MHC) class Ⅱ molecules in the EAE rat brain vascular endothelial cells were tested by immunohistochemistry. The level of tumor necrosis factor (TNF) and interferon\|γ(IFN\|γ) in defferent clinic grade EAE were tested by MTT [3\|(4 5\|dimethythiazol\|zyl)\|2\^5\|diphenyl) tetrazolium bromide] method. Results There were inducible expressions of VCAM\|1 and MHC class Ⅱ molecules in the EAE rat brain vascular endothelial cells compared with control. The sera of EAE rats possessed the activities of TNF and IFN\|γ.Conclusion\ In the CNS inflammation, the change surface molecules in the brain vascular endothelial cells may suggest that it plays important roles in EAE pathogenesis.\;[
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第2期215-218,共4页
Acta Anatomica Sinica
关键词
血管细胞粘附分子-1
相容性复合体Ⅱ类分子
自身免疫性脑脊髓炎
动物实验
Vascular cell adhesion molecule\|1(VCAM\|1)
Major histocompatibility complex class Ⅱ molecules (MHC class Ⅱ molecules)
Experimental autoimmune encephalomyelitis(EAE)
Rat
