摘要
目的观察急性CO中毒后大鼠脑内血管内皮细胞黏附分子-1(VCAM-1)表达、CD4+T淋巴细胞浸润情况,从系统细胞免疫反应角度探讨迟发性脑病(DNS)与免疫反应的关系。方法30只SD雄性大鼠随机分为对照组,染毒后1、3、7d组和高压氧(HBO)治疗7d组,每组6只。采用HE和免疫组织化学染色方法,观察染毒后各时间点大鼠脑内病理形态学变化,及VCAM-1表达、CD4+T淋巴细胞浸润情况。结果对照组无VCAM-1表达、无CD4+T淋巴细胞浸润;各染毒组大鼠脑内微血管内皮细胞和变性坏死的神经元均有VCAM-1不同程度的表达。染毒后1d组为表达高峰(P<0.01),3d组VCAM-1的表达数量有所减少。此时,局部大脑皮层开始有CD4+T淋巴细胞浸润;至染毒后7d,在部分变性坏死的神经元上仍有VCAM-1的持续表达,而CD4+T淋巴细胞浸润达高峰(P<0.01)。染毒后立即进行HBO治疗7d,其VCAM-1、CD4+T淋巴细胞均显著减少,与染毒后7d组相比差异有统计学意义(P<0.05)。结论系统细胞免疫反应可能参与了DNS的发生。
Objective To investigate the expression of Vascular Cell Adhesion Molecule - 1（ VCAM - 1 ） and the infiltration of CD4^ ＋ T lymphocytes in brains of acute Carbon Monoxide （ CO ） poisoned rats and study the relation of Delayed Neuropathological Sequelae {DNS） and immunoreaction through systemic cell immunoreaction . Methods CO - poisoned rats model was established in Twenty - four SD male rats and another six as control. The poisoned rats were equally divided into four groups ： 1, 3,7 d post - poisoning and 7 d post - Hyperbaric Oxygen（ HBO） treatment respectively. HE and Immunohistochemical staining were performed on frozen sections to observe the change of pathological morphous and the expression of VCAM - 1 and the infiltration of CD4^ ＋ T lymphocytes in brains of each group rats as compared with that of the control rats. Rrsults Immunohistochemical staining showed that there were expressions of VCAM - 1 in endothelial cells of cerebral microvessels and denaturatd neurons in poisoned group rats at a disparate degree, but no VCAM - 1 expression and CD4^＋ T lymphocytes infiltration were shown in control rats. VCAM - 1 expression reached the peak at lth d（ P 〈0.01 ） and became weak at 3th d and kept at 7th d, while CD4^＋ T lymphocytes initiated infiltration in poisoned rats partial cerebral cortex at 3th d and reached the peak at 7th d（P 〈0.01 ）. Moreover, the results of VCAM - 1 expression and CD4^＋ T lymphocytes decreased .greatly at HBO group and they both possessed significant statistical difference （ P 〈 0.05 ） respectively compared with that of 7th d post - poisoning. Conclusions Systemic cell immunoreaction was contributed to DNS possibly.
出处
《中国急救医学》
CAS
CSCD
北大核心
2007年第3期225-228,289,共5页
Chinese Journal of Critical Care Medicine
基金
全军"十一五"医药卫生课题(No06MA213)
