摘要
溴化氰是常用载体 (树脂 )活化剂 ,但其毒性很强 ,具有挥发性 ,活化后树脂带较强的电荷而导致非特异性吸附 .羰基二咪唑 (1,1′ carboxyl diimidazole,CDI)近年来被用于树脂的活化 ,具有毒性低、相对安全、方便、活化效率高、活化后树脂所带电荷相对较少等优点 .由于国内同行很少采用CDI活化树脂制备亲和层析柱或固定化酶 。
Cyanogen bromide has been widely used as an activator to some resins while preparing affinity chromatography column and immobilized enzymes. Nevertheless, it is a hazard poison with a volatilization feature, which should be carefully used. Here, 1, 1'-carboxyl-diimidazole is used as an activator to Sepharose CL-6B, in order to immobilize earthworm fibrinlytic enzyme III-1 (EFE-III-1), m-aminophenylboronic acid and to detect glycated D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), with a low poison, a high coupling rate and a convenient process.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2002年第2期328-331,共4页
Progress In Biochemistry and Biophysics
基金
北京市海淀区科委基金项目 (A0 6 1999 0 2 6 )~~
