摘要
目的 探讨磷酸甘油酸激酶 (PGK)位点克隆性分析技术对于确定局灶性或结节性病变的克隆组成的意义 ,以及子宫平滑肌瘤的克隆性及多发性肌瘤不同瘤结节之间的关系。方法 提取10 3例子宫平滑肌肿瘤新鲜组织DNA ,HpaⅡ消化 ,巢式聚合酶链反应扩增PGK基因 ,产物经BstⅪ消化后 ,琼脂糖凝胶电泳显示结果。结果 10 3例组织标本中 ,32例 (31% )具有BstⅪ酶切位点的多态性。对其中 2 9例共 89个瘤结节进行了检测 ,显示所有瘤结节均为单克隆性 ;1例多结节的平滑肌肉瘤中 ,检查过的 7个瘤结节均为同一PGK等位基因被灭活 ;而多发性子宫平滑肌瘤不同瘤结节之间的关系较复杂 ,其酶切带型可以完全相同 (8/15 )或大部分相同 (2 /15 ) ,也可以完全不同 (5 /15 )。这种差别与瘤组织核分裂象指数无关。结论 PGK克隆性分析技术可用于确定局灶性或结节性病变的克隆组成 ;子宫平滑肌瘤是单克隆起源 ;多结节的子宫平滑肌瘤可能有各自独立型、局部侵袭型以及二者的混合型三种。
Objective To study the clonality of uterine leiomyomas, especially the relationship between different nodules in multinodular cases. Methods Genomic DNA was extracted from fresh tissue samples and digested through incubation with Hpa II and amplified through nested polymerase chain reaction for phosphoglycerate kinase (PGK) gene. The products were treated with Bst Ⅺ and resolved on agarose gels. Results Among the 103 cases examined, 32 (31%) carried the polymorphic Bst Ⅺ site at the PGK locus. Eighty-nine tumors from the 29 cases were subjected to the cloning assay. Loss of polymorphism at the PGK locus was found in all tumor nodules, indicating the monoclonality of the tumor. The relationship between multiple tumors was also assessed by comparing their inactivated alleles. Seven nodules from a leiomyosarcoma were found to have originated from a single cell. However, the relationship was found to be more complicated, as demonstrated in 15 cases of multiple leiomyomas. The same inactivated allele was found in all nodules of 8 cases and in most nodules in 2 cases, while totally different inactivation patterns were observed in 5 cases. The difference was not associated with cell proliferation. Conclusions Clonality analysis can be applied to define the clonality of focal or nodular lesions. Uterine leiomyomas are of clonal origin. Multiple uterine leiomyomas may be subtyped into fully independent and aggressive types as well as a mixed type of both.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2002年第2期107-111,共5页
Chinese Journal of Pathology
基金
国家自然科学基金资助项目 ( 30 1710 5 2 )
国家回国人员研究启动基金资助项目 (Hg980 0 4)
